6. Henna Leaves

origin: the dried leaves of Lawsonia alba & other species   Bot.

F: Lythraceae

Active constituent:

1.     Lawsone, coloring substance of naphthaquinone structure.

2.     Tow coumarin derivatives, fat &

Henna tannin

Key element (microscopically examination):

1.     Paracytic &anomocytic stomata.

2.     Cluster calcium oxalate.

3.     The epidermal cells of upper and lower surfaces are identical (polygonal, tabular cells, straight or slightly wavy wall).

Uses:

1.     A natural red dye for cosmetic.

2.     Antifungal and antiseptic for skin &scalp.

                                                                                                                                                                    Test for identified:                                                                                                                              for lawsone: boil powder in 20ml of water for one minute filter, the filtrate is orange to orange brown in color.

1.     Add 2ml of dilute HCL to 2ml of the previous filtrate, the color fades away.

2.      Add 2ml of KOH T.S to 2ml of the filtrate a deep orange color is produced.

3.     Add 2ml of dilute ammonia solution to 2ml of the filtrate a titan red color is produced.

4.     Add 2ml of NaCO3 (5%) solution to 2ml of the filtrate an orange yellow color is produced.

Report in the lab.

&anomocytic stomata.	Paracytic stomata.
The epidermal cells of upper and lower surfaces	Cluster calcium oxalate.

                                     

Chemical test

Result	Test
	With dilute HCL
	With KOH T.S
	With dilute ammonia solution
	With NaCO3 (5%) solution

Conclusion:………………………………………………………………………………………………